Steps % Alcohol time Distilled water (ml) 95% Ethanol (ml) 100% Ethanol (ml) 100% TBA (ml)
1 50% for 2 h or more 50 40 0 10
2 70% overnight 30 50 0 20
3 85% 1-2 h 15 50 0 35
4 95% 1-2 h 0 45 0 55
5 100% 1-3 h 0 0 25 75
6 100% 1-3 h 0 0 0 100
7 100% 1-3 h 0 0 0 100
8 100% overnight 0 0 0 100
*TBA changes were carried out at 30°C
Table 1: Tertiary butyl alcohol dehydration schedule [7].