| Steps |
% Alcohol time |
Distilled water (ml) |
95% Ethanol (ml) |
100% Ethanol (ml) |
100% TBA (ml) |
| 1 |
50% for 2 h or more |
50 |
40 |
0 |
10 |
| 2 |
70% overnight |
30 |
50 |
0 |
20 |
| 3 |
85% 1-2 h |
15 |
50 |
0 |
35 |
| 4 |
95% 1-2 h |
0 |
45 |
0 |
55 |
| 5 |
100% 1-3 h |
0 |
0 |
25 |
75 |
| 6 |
100% 1-3 h |
0 |
0 |
0 |
100 |
| 7 |
100% 1-3 h |
0 |
0 |
0 |
100 |
| 8 |
100% overnight |
0 |
0 |
0 |
100 |
|
| *TBA changes were carried out at 30°C |
| Table 1: Tertiary butyl alcohol dehydration schedule [7]. |
