| Step |
Solution |
Time |
| 1. |
Xylene |
5 min |
| 2. |
Absolute ethanol |
5 min |
| 3. |
95% ethanol |
5 min |
| 4. |
70% ethanol |
5 min |
| 5. |
50% ethanol |
5 min |
| 6. |
30% ethanol |
5 min |
| 7. |
1% aqueous safranin O |
1-12 hr |
| 8. |
Rinse in tap water |
|
| 9. |
30% ethanol |
3 min |
| 10. |
50% ethanol |
3 min |
| 11 |
70% ethanol |
3 min |
| 12. |
95% ethanol |
3 min |
| 13. |
0.1% fast green FCF in 95% ethanol |
5-30 sec |
| 14. |
Absolute ethanol |
15 sec |
| 15. |
Absolute ethanol |
5 min |
| 16. |
Xylene-Absolute ethanol |
5 min |
| 17. |
Xylene |
5 min |
| 18. |
Xylene |
5 min or longer |
|
