Step |
Solution |
Time |
1. |
Xylene |
5 min |
2. |
Absolute ethanol |
5 min |
3. |
95% ethanol |
5 min |
4. |
70% ethanol |
5 min |
5. |
50% ethanol |
5 min |
6. |
30% ethanol |
5 min |
7. |
1% aqueous safranin O |
1-12 hr |
8. |
Rinse in tap water |
|
9. |
30% ethanol |
3 min |
10. |
50% ethanol |
3 min |
11 |
70% ethanol |
3 min |
12. |
95% ethanol |
3 min |
13. |
0.1% fast green FCF in 95% ethanol |
5-30 sec |
14. |
Absolute ethanol |
15 sec |
15. |
Absolute ethanol |
5 min |
16. |
Xylene-Absolute ethanol |
5 min |
17. |
Xylene |
5 min |
18. |
Xylene |
5 min or longer |
|