Step Solution Time
1. Xylene 5 min
2. Absolute ethanol 5 min
3. 95% ethanol 5 min
4. 70% ethanol 5 min
5. 50% ethanol 5 min
6. 30% ethanol 5 min
7. 1% aqueous safranin O 1-12 hr
8. Rinse in tap water  
9. 30% ethanol 3 min
10. 50% ethanol 3 min
11 70% ethanol 3 min
12. 95% ethanol 3 min
13. 0.1% fast green FCF in 95% ethanol 5-30 sec
14. Absolute ethanol 15 sec
15. Absolute ethanol 5 min
16. Xylene-Absolute ethanol 5 min
17. Xylene 5 min
18. Xylene 5 min or longer
Table 2: Safranin and Fast-Green Schedule [8].