Seed lot sampling method1 visual symptom2 pathogen DNA3 (Pg) per sample Uninfected seed At Random 0 0.18 Selective 1.2 0.26 Infected seed At Random 0.6 1.53 Selective 1.2 3.0 SED or Proportionate error In each mean   0.26 34%

1Seeds that were randomly selected or selected for symptoms of B. cinerea infection. 2Symptom visual scores; 0, no symptoms; 1, flecking symptoms; 2, lesions visible. Main effect of sampling method, P<0.001; seed lot and interaction P>0.1, not significant. 3Back-transformed mean; analysis on log scale (equivalent to the measurement scale). ANOVA: main effect of seed lot, P<0.001; sampling method and interaction P>0.2, not significant.

Table 2: Level of B. cinerea in clean (symptomless) or infected B. cinerea seed of various origins, determined by visual assessments and quantitative real-time PCR.