Figure 1: Inhibitory activity of CecA and derived peptides on Magnaporthe oryzae PR9 appressorium formation. (A) Appressorium formation of fungi treated with increasing concentrations of CecA for 6h at 28°C. (B) Quantification of different fungal developmental stages after 24h incubation with increasing concentrations of CecA. The developmental stages are appressoria (black), appressorium-like structures (grey) and germinated spore (white). (C) Relative appressorium formation (referred to control condition) after 6h incubation with 20 μM of CecA and its derived peptides at 28°C. The absolute value of formed appressoria in control condition and corresponding to 100% was between 75-85% in all the experiments. Asterisks denote statistically significant difference p<0.01, ANOVA. (D) Effect of two appressoria inducers on the inhibitory activity of CecA and MgAPI16. Conidia of M. oryzae PR9 were incubated alone (black), or with 2.4 mM cAMP (white) or 10 μM 1,16-hexadecanediol (grey) and in the absence or presence of 20 μM of each peptide on a hydrophilic surface for 6h at 28°C.