Antibody
Dilutions (v/v)
Antibody dilutions
H
I
II
III
IV
V
1:50
0.10
0.70
2.20
2.33
2.19
2.18
1:100
0.10
0.37
2.60
2.02
2.00
2.00
1:500
0.08
0.33
2.44
2.54
2.23
2.20
1:1000
0.08
0.25
2.30
2.50
2.19
2.10
1:5000
0.07
0.15
2.00
2.58
2.74
2.35
1:7000
0.07
0.10
0.95
2.34
2.40
1.98
1:10000
0.07
0.10
0.86
1.10
1.0
0.98
Table 2:
Titration of antiserum from different bleeds against healthy and PBNV-GN leaf antigens by DAC-ELISA. *A405 OD values are average values of duplicate wells