Figure 1: Rooted tree showing the phylogenetic relationships of RH-31, RH-32 and RH-33 with its closely related taxa on the basis of 16S rRNA gene sequence. The cluster analysis was performed having bootstrap value 500 using MEGA-5 software (a) RH-31; the tree was constructed on the basis of the neighbor-joining method using Thermobacillus xylanilyticus (AJ005795) as an out group. (b) RH-32; the tree was constructed on the basis of the neighbor-joining method using Bacillus cereus (AE016877) as an out group. (c) RH-33; the tree was constructed on the basis of the neighbor-joining method using Acinetobacter calcoaceticus (Z93434) as an out group.