suspension
Figure 1: (a) Potato suspension cell was infected with Phytophthora infestans (Pi) and the plasma membrane bound to infecting hyphae tip within 5min after the penetration of the cell wall. (b) The hypersensitive cell death was occurred in the suspension cells of cv. Rishiri (R1-gene) at 5 h after infection. The cell death was measured by loss of plasmolysis, loss of stainability by neutral red (5 mM), and granulation of cytoplasmic strands and particles in the cell. PM:Plasma membren of potato cell. Z: Zoospores of Pi. (c) Effect of hyphal cell wall elicitor and suppressor glucan on potato leaves (cv. Irish Cobbler, r-gene, and cv. Eniwa, R1-gene). The suppressor for hypersensitive response from Pi caused little effect on the leaves within 48 h after treatment, although elicitor caused severe HR responses on those ones.