Figure 2: (a) Effect of smGFP-CDPK overexpressing potato cells on the generation of AOS in the cells after PiP elicitor treatment at different time intervals (Control: without smGFP-CDPK1). : CLA was used as a chemiluminescent substrate for measuring the AOS generation by NADPH oxydase in potato cells by using luminometer. GFP::SdCDPK1+PiPE, Suspension cells (cv.Rishiri) +PiPE, GFP:: SdCDPK1, Suspension cells (cv. Rishiri). (b) smGFP-GFP-CDPK1 was transformed into the potato suspension cells by the particle gun. MF (5 μg) + NADPH oxydase- His (36 nM), MF (5 μg) + NADPH oxydase-His (3.6 nM), MF (5 μg) + NADPH oxydase-His (36 nM) + Tiron (10 mM), MF (5 μμg), MF (5 μg) + Tiron (10 mM). (c) Effect of insect cell expressed NADPH oxydase activity on the generation of AOS in potato membrane fraction (cv. Eniwa, R1-gene). CLA index showing generation of AOS in the suspension cells. Tiron was added for a specific scavenger of the AOS. MF (5 μg) + His-rboh1 (36 nM), MF (5 μg) + His-rboh1 (36 nM) + anti-p47phox and - antibodies(Abs) 67phox Abs, MF (5 μg) + His-rboh1 (36 nM) + anti-p67phox Abs, MF (5 μg) + His-rboh1 (36 nM) + anti-p47phox Abs, MF (5 μg). (d) Effect of predicted epitopes of p67phox and p47phox (NCF1) on generation of AOS in potato microsomal fractions. Predicted amino acid sequences of p67phox and p47phox. Amino acids showed are recognized amino acid residues by p67phox (DEPKESEKADANNQ) , and p47phox (PGPQSPGSPLEEERQ) and p47phox Abs. CLA assay of generation of AOS in potato microsomal fractions. MF+His-rboh1 (132μg/ml), MF+ His-rboh1 (132μg/ml)+ anti-p47phox Abs, MF+ His-rboh1 (132μg/ml)+ anti-p67phox Abs, MF+ His-rboh1 (132μg/ml)+ anti-p47phox and p67phox Abs, MF. (e) Effect of predicted antigen epitopes of CDPK on generation of AOS in potato microsomal proteins and His-rboh1. MF+ His-rboh1 (132μg/ml), MF+ His-rboh1 (132μg/ml)+ anti-CDPK-Abs, MF+ His-rboh1 (132μg/ml)+anti-CDPK-Abs +anti-p47phox and p67phox Abs, MF only.