suspension
Figure 6: Localization of CDPK in potato cells by means of fluorescent immunochemistry by using anti-CDPK-antibodies(Abs). (a) Potato suspension cells, which was taken by laser microscopy (bar means 10 μm). (b) The fluorescent microscopy shows localization of CDPK in potato cell. Note that potato cell was stain with the CDPK kinase domain I-Abs, in plasma membrane and on the cell wall. Some intracellular space, specially cytozol and on the surface of the nucleus. c, Model of CDPK signaling in potato activated by the suppressor of Phytophthora infestans (Pi). A membrane bound CDPK1 could control gp91phox activity because their phosphorylation by CDPK1 induced AOS generation in potato cells. The signaling cascade of the suppressor binding against the receptor CDPK in planta shows that the toxin from the pathogen induced CDPK signals to gp91phox, and then inhibit the hypersensitive response in compatible interaction. The suppressor inhibiting HR response are summarized on the basis of recent reviews [54,63, 64] from the pathogen. CDPK, calcium dependent protein kinase; N, nucleus.