genotype 2011/12 2012/13 2013/14 No. of virulent isolates No. of avirulent isolates Total No. of isolates Virulence frequency (%)* Level of varietal resistance (%)** No. of virulent isolates No. of avirulent isolates Total No. of isolates Virulence frequency (%) Level of varietal resistance (%) No. of virulent isolates No. of avirulent isolates Total No. of isolates Virulence frequency (%) Level of varietal resistance (%) Sakha 94 16 101 117 13.68 86.32 115 51 166 69.28 30.72 40 21 61 65.57 34.43 Sakha 95 61 57 118 51.69 48.31 110 55 165 66.67 33.33 25 31 56 44.64 55.36 Giza 168 75 43 118 63.56 36.44 148 18 166 89.16 10.84 47 14 61 77.05 22.95 Gemmeiza 7 84 34 118 71.19 28.81 120 46 166 72.29 27.71 45 16 61 73.77 26.23 Gemmeiza 9 91 25 116 78.45 21.55 103 63 166 62.05 37.95 48 12 60 80.00 20.00 Gemmeiza 10 102 16 118 86.44 13.56 118 48 166 71.08 28.92 44 17 61 72.13 27.87 Gemmeiza 11 40 74 114 35.09 64.91 102 64 166 61.45 38.55 48 13 61 78.69 21.31 Sids 1 74 44 118 62.71 37.29 106 60 166 63.86 36.14 38 17 55 69.09 30.91 Sids 12 13 105 118 11.02 88.98 29 137 166 17.47 82.53 14 47 61 22.95 77.05 Sids 13 7 110 117 5.98 94.02 88 78 166 53.01 46.99 44 17 61 72.13 27.87 Misr 1 2 115 117 1.71 98.29 9 157 166 5.42 94.58 8 52 60 13.33 86.67 Misr 2 10 107 117 8.55 91.45 17 149 166 10.24 89.76 35 26 61 57.38 42.62 Shandweel 1 52 65 117 44.44 55.56 71 95 166 42.77 57.23 44 17 61 72.13 27.87 No. of collected samples*** 40 38 29

Table 1: Percentage of virulence frequency of Puccinia triticina isolates and level of varietal resistance to thirteen Egyptian wheat genotypes during three successive growing seasons at seedling stage. *Virulence frequency (%)=Percentage of P. triticina isolates virulent to each wheat variety to the total number of tested isolates. ** Level of varietal resistance (%): Estimated as the percentage of virulent isolates to the total number of tested isolates.