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Figure 2: Anti-Xoo antibody specificity evaluation using a WB analysis. Total proteins were isolated from uninfected rice (TP309), E. coli (DH5α), Xoo (P2, P6, P8, P9, and P10), and M. oryzae (TH12). SDS-PAGE separated proteins were transferred to PVDF membranes, assayed using (A) the anti- Xoo antibody, and (B) a parallel gel was stained with Coomassie blue. |