mixed
Figure 3: Detection of the Xoo protein when mixed with rice samples using a WB analysis.
A) Total Xoo proteins were mixed with samples isolated from rice TP309 and separated by SDS-PAGE. A WB analysis was performed using the anti-Xoo antibody. HSP: The abundance of HSP protein in rice was determined and used as a loading control.
B) Quantification of signal intensity collected from three repeats of WB analysis. The average and standard derivations were calculated. The standard curve was generated and a linear regression was performed.