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Figure 3: Detection of the Xoo protein when mixed with rice samples using
a WB analysis. A) Total Xoo proteins were mixed with samples isolated from rice TP309 and separated by SDS-PAGE. A WB analysis was performed using the anti-Xoo antibody. HSP: The abundance of HSP protein in rice was determined and used as a loading control. B) Quantification of signal intensity collected from three repeats of WB analysis. The average and standard derivations were calculated. The standard curve was generated and a linear regression was performed. |