A) WB detection of Xoo levels in rice leaves at different time points after inoculation. At 1, 2, 3, 4, and 5 days after Xoo inoculation total proteins were isolated from rice leaves. SDS-PAGE separated total proteins were transferred to PVDF membrane and incubated with an anti-Xoo antibody. HSP: The abundance of HSP protein in rice was used as a loading control.
B) Quantification of Xoo levels detected using WB. The signal intensities were collected from three independent WBs and normalized based on the total signal intensities within a gel. The averages and standard derivations were calculated. R: Samples collected from Xoo-inoculated 4021 rice plants; S: Samples collected from Xoo-inoculated TP309 rice plants.
