Figure 1: Cytotoxicity of 1,25(OH)2D3, (1,25(OH)2D3)L, 1,25(OH)2D3-3-BE or (1,25(OH)2D3-3-BE)L in LNCaP cells. Cells were treated with ethanol (control), blank liposome (control), various doses of 1,25(OH)2D3, (1,25(OH)2D3) L, 1,25(OH)2D3-3-BE or (1,25(OH)2D3-3-BE)L for 16 hr. Metabolic activity in reagent-treated cells relative to controls was quantitated using a tetrazoliumbased (MTT) chromogenic assay. OD was measured spectrophotometrically at 450 nm. There were six replicates for each sample. Statistics was done by studentís t test: ***p<0.001, **p<0.01.