Figure 5: Evaluation of the serum-stability of (1,25(OH)2D3-3-BE)L. One ml of human serum was incubated at 37°C for 60 minutes with a sample of (1,25(OH)2D3-3-BE)L (10 µg) followed by extraction with ethyl acetate and HPLC-analysis of the organic-extract. A standard sample of 1,25(OH)2D3-3-BE was also analyzed by HPLC under same conditions. Inset: Graphic depiction of hydrolytic production of equimolar amount of 1,25(OH)2D3 and bromoacetic acid from 1,25(OH)2D3-3-BE.