Figure 1: Effect of transfection with human p21 cDNA on the accumulation of porcine granulosa cells containig protein p21. Monolayer of granulosa cells of prepubertal gilts were transfected with p21 gene construct and after 30 hours of incubation analyzed by SDS-PAGE-Western immunoblotting. Molecular weight is indicated (kDa). Loading control GAPDH is not shown.