Sequence   Nucleotide Position   PCR Product Size
 
Detection Primers      
GR-α

(FW)

 5′-TGTTTTGCTCCTGATCTGA-3′ 1982 to 2000 ∼389bp
 

(BW)

 5′-TCGGGGAATTCAATACTCA-3′ 2352 to 2370
 

     
GR-β

(FW)

 5′-TGTTTTGCTCCTGATCTGA-3′ 1996 to 2014 ∼383bp
 

(BW)

 5′-TGAGCCCAAGATTGT-3′ 2373 to 2388
 

     
Nested GR-β Primers    
Round 1

(FW)

 5′-CCTAAGGACGGTCTGAAGAGC-3′ 2144 to 2164 ∼366bp
 

(BW)

 5′-CCACGTATCCTAAAAGGGCAC-3′ 5119 to 5139
 

     
Round 2

(FW)

 5′-AGCACATCTCACACATTAAT-3′ 4954 to 4973 ∼134bp
 

(BW)

 5′-TATAGTTGTCGATGAGCATC-3′ 5071 to 5087
       
Housekeeping Gene Primers    
GAPDH

(FW)

 5′- CTGCCGTCTAGAAAAACC-3′ 840 to 857 ∼220bp
 

(BW)

 5′- CCAAATTCGTTGTCATACC-3′ 1039 to 1058
 

     
FW, forward; BW, backward
GR primer location according to GenBank accession number NM_001018076 sequence; GAPDH according to GenBank accession number sequence NM_002046
Table 1: Primers used for semi-quantitative RT-PCR.