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Figure 1: Modulation by hyperglycemia of the expression of mRNA for ERa and ERb in primary human female- derived osteoblasts. Bone cells were obtained and cultured, and extracts prepared for real time PCR analysis as described in Materials and Methods. Results are means SEM for triplicate cultures from 5 donors for each group. Means of cells grown in high glucose (grey bars) were compared to cells grown in normal glucose (open bars): *, P <0.05; **, P <0.01. |