Figure 2: Stimulation of CK specific activity by different phytoestrogens and E2 in primary bone-derived cells, from pre- and post- menopausal women, grown at different glucose concentrations; either normal (NG; 22mM) or high (HG; 44mM gray bars) glucose concentrations. Bone cells were cultured, treated and assayed for CK activity as described in Materials and Methods. Cells were treated for 24h with vehicle or 30nM E2 or 300nM of cD, Gla or Glb or 3000nM of D. Results are means SEM for triplicate cultures from 5 women/ group for each group. Control means of CK specific activity were 28.66.5 and 24.64.0nmol/min/mg protein, for pre- and post-menopausal women respectively. Experimental means compared to control means: *, P <0.05; **, P < 0.01; ***, P < 0.001. Means of cells at HG+ estrogenic compounds vs. cells at NG+ estrogenic compound: #, P <0.05; ##, P < 0.01.