Figure 4: Modulation of the specific binding of estrogenic compounds to nuclear binding sites in primary bone-derived cells from pre- and post- menopausal women, grown at different glucose concentrations either normal (NG; 22mM) or high (HG; 44mM gray bars) glucose concentrations. Bone cells were cultured, treated and assayed for nuclear binding of 3[H] E2 as described in Materials and Methods. Results are means ± SEM for % of specific binding of triplicate cultures in the presence of 500 fold excess concentration of the different phytoestrogens as competitors. Experimental means compared to control means: *, P < 0.05.