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Figure 5: Modulation of the specific binding of estrogenic compounds to membranal binding sites in primary bone-derived cells from pre- and postmenopausal women, grown at different glucose concentrations either normal (NG; 22mM) or high (HG; 44mM gray bars) concentrations. Bone cells were cultured, treated and assayed for membranal binding of Eu- Ov- E2 as described in Materials and Methods. Results are means ± SEM for % of specific binding of triplicate cultures in the presence of 500 fold concentration of the different phytoestrogenic-protein conjugates as competitors. Experimental means compared to control means: *, P < 0.05. |