Figure 6: Modulation of the expression of mRNA for 1-OHase (lower panel) and the production of 1,25D (upper panel) in cultured bone cells from pre- and postmenopausal women by high glucose (HG, dark gray bars) in the growth medium compared to normal glucose (NG, light gray bars). Conditions for the different parameters and the assays are as described in Materials and Methods. Results are expressed as % change in the concentration of 1,25D, (pg/mg protein) or in 1- OHase mRNA expression as quantified by real time PCR (n=4-8). *P<0.05; **p<0.01, compared with growth in NG.