Figure 1: Regulation of expression of SLPI by steroid hormone in BeWo cells. A & B: Effect of addition of E2 or Progesterone and C&D: Different concentrations of progesterone. (A) Cells were incubated with 1 µM P4 or 2.5µM E2 for a period of 12h. RNA isolated was reverse transcribed and cDNA was subjected to semi-quantitative PCR in the linear range of amplification with GAPDH as an internal control. B) Graphical representation of results presented in A, after normalizing to GAPDH used as an internal control (values expressed as fold increase over control mean ± S.E. from three independent experiments). C) Cells were incubated with different concentrations of P4 ranging from 0.01µM to 1µM for 12h. RNA was reverse transcribed and cDNA subjected to semi-quantitative PCR in the linear range of amplification. D) Graphical representation of results presented in C, (values expressed as fold increase over control mean ± S.E. from three independent experiments). * Significantly different from Control p<0.001.