Figure 1: Experimental design: Control, 25 mM and 50 mM groups were groups were grown separated flasks during all the sustained exposure. Every five passages we obtained three subculture replicates of each group to carry out the experiments, at a seeding density of 0.1×106 viable cells/ml in 100 mm- plate. Cells and culture medium of these replicates were harvested after 1 h, 24 h and 72 h of the last ethanol exposure.