Figure 5: Anterior Gradient Protein 2 (AGH-2) protein and mRNA levels increase in mice cervix during pregnancy compared to non-pregnancy, as revealed by proteomics (A) and confirmed by real time PCR (B). No difference was noted in AGH-2 protein levels between early (day 11) and late (d 17) pregnancy (A). However, mRNA levels were lower in early compared to late pregnancy (B). (n=3 and 5 for proteomic and real time PCR analyses, respectively; p<0.05 for NP vs. day 11/day 17; Figure 4B: NP vs. day 11/17 and day 11 vs. day 17).