| System |
Parameter |
Setting |
| Autosampler |
Temperature |
4°C |
| |
Injection |
5.0µL prepared sample |
| LC Trap Column |
Sample load time |
5 min |
| |
Trap flow rate |
5 µL/min |
| LC Analytical Column |
Column temperature |
55 °C |
| |
Flow rate |
0.4µL/min |
| |
Gradient |
Initial conditions: 98% A |
| |
|
t = 60 min, 60% A |
| |
|
t = 65 min, 5% A |
| |
|
t = 70 min, 5 % A |
| |
|
t = 75 min, 98 % A |
| |
|
t = 95 min, 98 % A |
| LTQ Orbitrap XL Mass
Spectrometer |
Experiment type |
Nth Order Double Play |
| |
# Dependent scans |
5 |
| |
Source voltage |
2.0 kV |
| |
Capillary temperature |
200 °C |
| |
Source current |
1.0 µA |
| |
Capillary voltage |
47.0 V |
| |
Tube lens |
100.0 V |
| |
FTMS survey scan time |
1.0 sec |
| |
FTMS resolution |
60,000 |
| |
Survey time |
90 min |
| |
Survey ion mode |
ES Mode |
| |
Survey polarity |
Positive |
| |
Survey mass range |
375-2000 |
| |
Activation type |
CID |
| |
Normalized collision energy |
35.0 |
| |
ITMS MS/MS mass range |
50-2000 |
| |
ITMS MS/MS scan time |
0.2 sec |
|
