Median staining intensity (score)
  PKC a a1-Na,K-ATPase
  sham Aldo sham Aldo
Cortex        
Glomerulus 3 4 0 0
PCT 1 3 2 3
DCT 1 0 4 4
CCD 2 0 4 4
Pcap 1 2 1 1
Outer medulla        
Outer stripe        
TALH 3 4 4 4
MCD 3 4 4 4
PTs 1 2 3 2
Inner stripe        
TALH 1 2 4 4
MCD 1 3 2 3
VR 3 4 1 1
tLH 3 4 1 1
Inner medulla        
MCD 4 2 3 4
VR 4 4 1 1
tLH 3 4 1 1
Staining intensity: 0: negative, no reactivity; 1: trace, faint, or pale brown staining with less membrane reactivity; 2: weak, light brown staining with incomplete membrane reactivity; 3: moderate, shaded of brown staining of intermediate darkness with usually almost complete membrane reactivity; 4: strong, dark brown to black staining with usually complete membrane pattern, producing a thick outline of the cell [10].
PCT: Proximal Convoluted Tubule; DCT: Distal Convoluted Tubule; CCD: Cortical Collecting Duct; Pcap: Peritubular capillary; TALH: Thick Ascending Limb of Henle’s Loop; MCD: Medullary Collecting Duct; PTs: Proximal Straight Tubule; VR=Vasa Recta; tLH: thin limb of Henle’s loop.
Table 1: Median staining intensity (score) of renal PKC α and α1-Na,K-ATPase protein expressions.