Figure 4: Immunohistochemistry and mRNA expression of ERα and PR in the rat uterus after treatment with vehicle control, genistein, kaempferol, and genistein+kaempferol for 8 days. Kaempferol and genistein differentially regulate PR and ERα mRNA, protein expression, and localization in the uterus. qPCR analyses were performed using SYBR technologies (A) and uterine sections were stained for ERα (B) and PR (C) expression, n=8 per group. Bar=100 μm. All results were normalized to RPL1. Mean ± SEM. *p < 0.05, significantly different from control. Groups unmarked were not statistically different from each other.