Figure 5: Regulation of PR protein expression in human endometrial stromal cells. Cells were incubated with pure compound for 48 hours. PRA and PRB were induced by E2 (1 μM), genistein (5 μM) and kaempferol (20 μM) as determined by densitometry (A). PR fold change was analyzed using Image-J in triplicate experiments. *p < 0.05, indicates significant fold change of PR compared to basal DMSO. MPA (20 μM) did not induce PRA and PRB. Membranes were blotted for actin as a loading control (B). Image is a representative blot, experiment repeated in triplicate.