Figure 7: PSA-luciferase induction in MDA-MB-231 cells. MDAMB- 231 cells were transiently transfected with PSA-luciferase and treated with pure compounds kaempferol (20 μM), P4 (1 μM), and MPA (1 μM)) with and without AR antagonist RU486 (1 μM) for 48 hours. MPA activates PSA-luciferase and can be antagonized by RU486. Kaempferol and P4 did not significantly activate AR signaling. Data represent mean fold change ± SEM of relative light units normalized to β-gal in triplicate experiments. (a) indicates significant luciferase induction compared to basal DMSO; (b) indicates significant reduction of luciferase induction by RU486 as determined by Student’s t-Test, *p < 0.05.