Figure 1A: Slc2a1 gene expression is not regulated in the pituitary gland of mice. Pituitary glands were isolated from pre-pubertal and adult C57BL/6J male mice. Pituitary RNA was prepared for realtime PCR analysis with Taqman probes for Slc2a1 , Lhb and Tbp genes. Fold effects were determined using pre-pubertal mice as the control. Pre-pubertal males (white bars, n=7-9), and adult males (black bars, n=12) were used for Slc2a1 and Lhb gene expression. Significance was determined by Student’s t-test as shown by * indicating P <0.05.