Figure 4a: SLC2A1 protein is regulated by estradiol in gonadotrope cells. RNA was isolated cells for real-time PCR analysis to examine changes in Glut1 gene expression. Fold effects were determined by comparing SLC2A1 levels in vehicle alone (ethanol, 0 hr) compared to steroid-treated cells. The graph represents the mean±S.E.M (n= 2 for each time point).