Figure 4b: SLC2A1 protein is regulated by estradiol in gonadotrope cells. After LβT2 cells were treated with estradiol and/or progesterone as described above, whole cell extracts were isolated for Western blot analysis with SLC2A1 and α-tubulin antibodies. The α-tubulin antibody was used as a loading control to normalize protein. A picture of a representative autoradiograph is shown. Densitometry analysis was performed and SLC2A1 protein was normalized to α-Tubulin protein and then to control (unstimulated, 0hr) cells to determine fold effects. The mean ±S.E.M (n=4) is shown in the graph. Significance was determined using ANOVA and * indicates P<0.05.