Figure 1: CCC chromatogram of total steroid saponins. Solvent system: ethyl acetate-n-butanol-methanol-water (4:1:2:4, v/v); stationary phase: upper organic phase; mobile phase: lower aqueous phase; flow-rate: 2.0 ml min-1; revolution speed: 850 rpm; sample: 100 mg dissolved in 10 ml lower phase; retention of the stationary phase: 42%; Alltech 800 ELSD condition: drift tube temperature: 55°C; gas flow rate 3.0 L min-1.