Figure 3: HPLC chromatograms of total saponins, CCC and pre- HPLC peak fractions. Conditions: column: Welchrom C18 column (250x4.6 mm, 5 μm); mobile phase: acetonitrile-water (30:70, v/v); flow rate: 1 ml min-1; Alltech 2000 ELSD condition: drift tube temperature: 104°C; gas flow rate 2.8 L min-1; column temperature: 25°C; injection volume: 10 μL. (a): Total steroid saponins; (b): Compounds A; (c): Compounds B; (d): Compounds C; (e): Compounds D; (f): Compounds E.