| Two-phase solvent systems |
Ratio(v/v) |
The final retention of stationary phase |
|
K |
|
| A |
B |
C |
D |
E |
| Chloroform-methanol-water |
4:03:02 |
- |
22.2 |
132.89 |
25.45 |
35.1 |
37.2 |
| Chloroform-n-butanol-methanol-water |
4:0.2:3:2 |
- |
136.4 |
27.5 |
14.2 |
33.1 |
35.9 |
| n-hexane-ethyl acetate-methanol-water |
1:1:1:1 |
- |
- |
- |
- |
- |
- |
| Ethyl acetate-n-butanol-water |
4:01:05 |
- |
0.16 |
0.02 |
0.08 |
0.32 |
0.41 |
| Ethyl acetate-n-butanol-methanol-water |
4:1:0.5:6 |
- |
0.21 |
0.04 |
0.14 |
0.41 |
0.53 |
| Ethyl acetate-n-butanol-methanol-water |
8:0.5:2:4 |
28% |
0.28 |
0.1 |
0.19 |
0.55 |
0.72 |
| Ethyl acetate-n-butanol-methanol-water |
7:0.5:2:4 |
30% |
0.32 |
0.14 |
0.24 |
0.84 |
1.03 |
| Ethyl acetate-n-butanol-methanol-water |
6:0.5:2:4 |
32% |
0.4 |
0.21 |
0.54 |
1.03 |
1.52 |
| Ethyl acetate-n-butanol-methanol-water |
4:1:2:4 |
42% |
0.49 |
0.98 |
1.23 |
1.58 |
1.97 |
| Ethyl acetate-n-butanol-methanol-water |
4:2:2:4 |
45% |
0.53 |
1.15 |
1.74 |
1.98 |
2.35 |
