Various aspects CCC preparative RPHPLC Separation mechanism Liquid-liquid mode Liquid-solid mode Irreversible adsorption None Some Cycle time of each separation Long Short Choice of solvent system Many Few Solid phase types None Many Elution mode Few Several Sample preparation Simple Complex Optimizing the chromatographic condition Complex Complex Sample loading capacity Large Small Effluent monitoring method Selecting solvent system Solvent consumption Amplification from the analytical mode Separation efficiency Recoveries Few Time-consuming Low Relatively simple A little better higher Many Relatively simple High Time-consuming A little worse Lower

Table 3: Comparison of CCC and preparative RP-HPLC.