Parameters CCC RP-HPLC Ratio CCC/RP-HPLC
Run time/injection (min) 210 60 3.5
Mobile phase flow rate (mL min-1) 2 16 0.125
Operating pressure (Mpa) 2 19.97 0.1
Column volumes (mL) 300 60 5
Sample loading/run (mg) 100 100 1
Injection volume (mL) 10 2 5
Total solvent used 420 960 0.437
Purity of five compounds Over 95% Over 95% Close 1
Detector ELSD UV -
Table 4: Comparison of performance parameters between CCC and RP-HPLC chromatography for 100 mg steroid saponins separation campaigns.