Figure 2: PPARγ agonists oppose estradiol in modulating expression of key regulators of growth in breast cancer cells. A, Real-time PCR analysis of endogenous CXCL12, Cyclin D1 and p21 expression or B, endogenous pS2, PR and ID3 expression in MCF-7 cells. MCF-7 cells were grown for 48 h in phenol-red free DMEM + 10% stripped FCS and then treated with vehicle (Veh) (ethanol), 17β-estradiol (E2) (10 nM), rosiglitazone (Rosi) (1 μM), or E2+Rosi. After 24 h, cells were harvested for total RNA, which was used for real-time PCR. Graphical data are represented as fold induction over vehicle (set at 1). Data points represent the average of triplicate amplification reactions for each condition in a representative experiment.