Figure 5: GW7845 manifests the anti-proliferative effects of PPARγ without inducing the proliferative effects of PPARγ. (A) MCF-7 and ZR-75 cells were seeded in 96-well plates and treated with vehicle (Veh), 17β-estradiol (E2) (10 nM), GW7845 (100 nM), or E2+GW7845 for 96 h. Cells were harvested for proliferation assays. Graphical data are represented as % increase in cell number over vehicle (set at 100%). Data points represent the average of quadruplicate samples for each condition in a representative experiment. (B) MDA MB-231 cells were seeded in 96-well plates and treated with vehicle (Veh), carbaprostacyclin (Carb) (1 μM), rosiglitazone (Rosi) (100 nM or 10 μM), GW7845 (10 nM or 1 μM) in the absence or presence of GSK660 (1 μM) for 96 h. Cell proliferation (quadruplicate samples per treatment) was assayed and analyzed as above.