Table 1

Cell Line

PR(t)

PR(p)

mPR-α (t)

mPR-β (t)

PGRMC1 (t)

ERα (t)

ERα (p)

ER β (t)

ERβ (p)

GPR30 (t)

GRP30 (p)

AR (t)

AR (p)

GN11

GT1-7

HN33

HT22

+++

HT22*

+++

NLT

SN56

+++

+= receptor level expression, t = mRNA transcript, p = protein expression
This data was obtained from our laboratory and all cell passage numbers were less than 20.
Culture condition for each cell type: GN-11 (DMEM + 10% fetal bovine serum (FBS) + 1% penicillin- streptomycin (PS), GT1-7 (DMEM + 10% FBS + 1% PS), HN33 (DMEM + 10% FBS + 1% PS), HT22 (DMEM + 10% charcoal-stripped FBS + 20µg/ml gentamycin). HT22* (DMEM + 10% FBS + 1% PS), NLT (DMEM + 10% FBS + 1% PS), SN56 (DMEM + 10% FBS + 1% PS).

Table 1: Steroid Hormone Receptor Profile for Forebrain Cell Lines.