Cell Line |
PR(t) |
PR(p) |
mPR-α (t) |
mPR-β (t) |
PGRMC1 (t) |
ERα (t) |
ERα (p) |
ER β (t) |
ERβ (p) |
GPR30 (t) |
GRP30 (p) |
AR (t) |
AR (p) |
CAD |
++ |
|
++ |
- |
++ |
|
+ |
|
|
|
|
|
|
N27 |
- |
|
|
|
|
+ |
+ |
+ |
|
|
|
- |
+ |
N2A |
|
|
|
|
|
++ |
+ |
++ |
+ |
+++ |
|
|
|
PC-12 |
++ |
|
+++ |
++ |
+++ |
- |
|
+ |
|
|
|
- |
|
SH-SY5Y |
- |
|
- |
- |
|
- |
|
- |
|
|
|
|
|
+= receptor level expression, t = mRNA transcript, p = protein expression
This data was obtained from our laboratory and all cell passage numbers were less than 20, except PC-12. PC-12 cells were passaged between 80-90. Culture condition for each cell type: CAD (DMEM/F12 + 8% FBS + 1% PS), N27 (RPMI +10% FBS + 1% PS), N2A (MEM + 10% FBS + 1% PS), PC-12 (RPMI + 5% FBS + 10% heat inactivatded horse serum + 1% PS), SH-SY5Y (DMEM/F12 + 10% FBS + 1% PS). |
