pH

Δn

ΔHint (kcal/mol)

Kan B

7.1

0.16 ± 0.12

-17.3 ± 0.9

Kan B

7.6

1.3 ± 0.3

-25.6 ± 1.7

Kan B

8.1

1.1 ± 0.2

-25.8 ± 1.2

Kan A

7.1

0.45  ± 0.15

-19.7 ± 1.8

Kan A

7.6

1.4 ± 0.2

-24.4 ± 0.6

Kan A

8.1

 0.90  ± 0.2

-24.3 ± 2.8

Neomycin

7.1

-0.22  ± 0.14

-10.0  ± 1.3

Neomycin

7.6

1.0  ± 0.3

-18.9 ± 1.9

Neomycin

8.1

2.0 ± 0.2

-31.5 ± 1.1

Table 1: pH-dependent protonation in the binary ANT-Aminoglycoside complexes