parameters

First binding sites

Second binding sites

       p

1

1

        gi

6.00±0.18

8.00±0.12

        Ka / M-1

2760993.60±49.00

928352±16.18

ΔH/ kJ mol-1

-19.90±0.04

-18.38±0.03

ΔG/ kJ mol-1

-36.71±0.09

-34.01±0.11

ΔS / kJ mol-1K-1

0.056±0.002

0.052±0.002

-0.31±0.03

 

 

1.07±0.09

Table 2: Binding parameters for ASP+HSA interaction recovered from Eqs.1 and 2 at pH 7 and 298K. p=1 indicates that the binding is non-cooperative in the two sets of binding sites. The positive value of indicate that ASP+HSA complex is stable and the negative value of imply that aspirin destabilizes the HSA structure due to its interaction with –CO and –NH2 groups of amino acids (non-specific interactions) in the low aspirin concentration region. The interaction of aspirin with HSA is strong as indicated by big equilibrium association constants.