Figure 4:Effect of pH on the binding constant (Kb) of kanamycin A (panel A, filled circles) and kanamycin B (panel B, filled circles) to ANT. Determined Δn (Δ) is shown with the overall charge on aminoglycosides ([NH3 +]- open
squares) and the total charge ([NH3 +]+Δn, open diamonds) at three different pH.