Figure 1: Intracellular delivery of non-viral DNA lipoplexes. The process is initiated with the binding of the lipoplex to the cell surface (A), followed by internalization into endosomes (B). Endosomal escape of the lipoplex(C) is necessary for the subsequent release of the plasmid with dissociation of the carrier molecules and uptake into the nucleus (D). Transcription of the therapeutic gene in the nucleus (E) is followed by its translation in the cytoplasm (F) and expression of therapeutic protein (G). Reproduced from Nanomedicine, Volume 5, Issue 7, pp.1103-1127 with permission of Future Medicine Ltd.