Figure 2: Carbon production observed through hydrolysis of amorphous cellulose (35 mg/mL). a) Hydrolysis of cellophane paper using free cellulase (C1) and immobilized cellulases (C1S1, and C1S2); b) As described in a), but using C2, C2S1 and C2S2; c) Hydrolysis of phosphoric acid swollen cellulose (PASC) using free cellulase (C1) and immobilized cellulases (C1S1 and C1S2); d) As described in c), but using C2, C2S1 and C2S2. Hydrolysis reactions were conducted in 0.1 M phosphate buffer (pH 5.0) at 50°C for 24 h using a water bath; n = 3; Mean ± Standard Error. Values that were significantly higher or lower (p<0.05) than those obtained using the corresponding free cellulase are denoted with an asterisk (*) and double asterisk (**), respectively.