Figure 3: DSC unfolding curves of cUNG and nine cUNG mutants with amino acid substitutions in the Leucine-loop. The transition curves were obtained at 1°C/min scan rate in a buffer [25 mM Hepes, 10 mM NaCl, 1 mM EDTA, 1% (v/v) glycerol, pH 7.4]. The thermograms are baseline-subtracted and normalized for protein concentration.