| Mutant |
5’-primer |
| A266T |
CATGTCTTGCAAACCGTTCATCCATCTC |
| V267A |
CATGTCTTGCAAGCTGCGCATCCATCTCCTTTG |
| Double (A266T/V267A) |
CATGTCTTGCAAACCGCGCATCCATCTCCTTTG |
| A274V |
CCTTTGTCTGTGCATCGTGGGTTCCTTGGTTG |
| Triple1 (A266T/V267A/A274V) |
GCAAACCGCGCATCCATCTCCTTTGTCTGTGCATCGTG |
| H275Y |
GTCTGCTTATCGTGGGTTC |
Leuloop
(A266T/V267A/A274V/H275Y) |
CTTGCAAACCGCGCATCCATCTCCTTTGTCTGTGTATCGTG |
| L279F |
CCTTTGTCTGCTCATCGTGGGTTCTTTGGTTG |
| Triple2 (A274V/H275Y/L279F) |
CCTTTGTCTGTGTATCGTGGGTTCTTTGGTTG |
