UNG 37°C 22°C 15°C
cUNG 0 0 0
A266T -9 -2 9
V267A 4 38 47
Double (A266T/V267A) -40 14 2
A274V 24 52 19
Triple1 (A266T/V267A/A274V) -26 -25 -32
H275Y 52 51 44
Leuloop
(A266T/V267A/A274V/H275Y)
46 57 46
L279F 36 51 28
Triple2 (A274V/H275Y/L279F) 43 71 63
hUNG 48 48 49
Table 3: Reduction (%) in kcat at different temperatures for cUNG mutants and hUNG relative to cUNG.